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Image Search Results
Journal: Nature Communications
Article Title: A broadly protective therapeutic antibody against influenza B virus with two mechanisms of action
doi: 10.1038/ncomms14234
Figure Lengend Snippet: ( a ) A549 cells infected with B/Brisbane/60/2008 were labelled with 46B8, Rituximab (negative control) or Cetuximab (positive control) prior to incubation with NK cells. NK cell activation (left panel) and target cell lysis (right panel) were presented as the frequency of CD107a + cells and the percent of LDH release, respectively. ( b ) A549 cells infected with B/Brisbane/60/2008 were labelled with 46B8 or 46B8 N297G prior to incubation with NK cells. Target cell lysis was presented as the percent of LDH release. The assays were done in duplicate. Results are representative of three independent experiments. ( a , b : mean and s.e.m.)
Article Snippet: For CDC assay,
Techniques: Infection, Negative Control, Positive Control, Incubation, Activation Assay, Lysis
Journal: Nature Communications
Article Title: A broadly protective therapeutic antibody against influenza B virus with two mechanisms of action
doi: 10.1038/ncomms14234
Figure Lengend Snippet: ( a ) A549 cells infected with WT or mutant B/Brisbane/60/2008 viruses were labelled with 46B8 prior to incubation with NK cells. NK cell activation (left panel) and target cell lysis (right panel) were presented as the frequency of CD107a + cells and the percent of LDH release, respectively. The assays were done in duplicate. Results are representative of three independent experiments. ( b , c ) DBA/2 J mice were infected intranasally with a minimum lethal dose of the WT or mutant B/Brisbane/60/2008 viruses. At 72 h post infection, mice received a single treatment of 46B8, 46B8 N297G or a control IgG intravenously at 15 mg kg −1 . ( b ) Survival curves. Each group contains eight mice. Log-rank tests of 46B8- versus control IgG-treated groups for all viruses, 46B8 N297G- versus control IgG-treated groups for WT, B1 and C2 and 46B8- versus 46B8 N297G-treated groups for A4, B1 and C2 give P <0.05. ( c ) Percent of average BW of survived mice as compared with the average pre-infection weight. ( a : mean and s.e.m.; c : mean and s.d.)
Article Snippet: For CDC assay,
Techniques: Infection, Mutagenesis, Incubation, Activation Assay, Lysis, Control